A simple flow-injection method for the determination of blood glucose using a Technicon immobilized enzyme coil
نویسنده
چکیده
The applicability of a single-channel flow-injection system with immobilized enzyme coil (Technicon) and UV detection to the determination of glucose is described. The method was used for a pure glucose solution and for serum. The detection limit was 0.10 mM, the rate of determination was 20-40 per hour and the precision was satisfactory. The system is very simple and practical when many analysis are to be determined periodically.
منابع مشابه
Continuous-flow Enzymatic Determination of Creatinine with Improved On-line Removal of Endogenous Ammonia
A new continuous-flow automated enzymatic method suitable for the direct determination of creatinine in physiological samples is described. The proposed system utilizes an on-line gas predialysis unit in conjunction with a flow-through enzyme reactor coil and a potentiometric ammonia detector. The enzyme reactor contains immobilized creatinine iminohydrolase (EC 3.5.4.21) which converts creatin...
متن کاملThe use of immobilized enzyme reactors in continuous-flow analyzers for the determination of glucose, urea, and uric acid.
We describe the use of immobilized enzymes in assay methods for the determination of glucose with glucose oxidase, uric acid with uricase, and urea with urease in serum samples. The enzyme reactor tubes were adapted to continuous-flow analyzers (Technicon AA II, SMA 12/60, and SMAC) used in routine laboratory determinations, and results with their use were compared to those from assays involvin...
متن کاملA Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots
Glucose, as the major energy source in cellular metabolism, plays an important role in the natural growth of cells. Herein, a simple, rapid and low-cost method for the glucose determination by utilizing glucose oxidase and CdTe/thioglycolic acid (TGA) quantum dots (QDs) on a thin layer chromatography (TLC) plate has been described. The detection was based on the combination of the glucose enzym...
متن کاملImmobilized-enzyme nylon-tube reactors for routine determination of urea and citrulline in serum.
A continuous-flow clinical analyzer for the routine estimation of urea is described that makes use of an immobilized-enzyme nylon-tube reactor as part of a flow-through system (a Technicon AutoAnalyzer I). Results of blood-urea analyses by use of the immobilized urease are compared with determinations made with the diacetyl monoxime method and the urease solution method. Clinical trials carried...
متن کاملDevelopment of An Immobilized Enzyme Electrode for the Determination of Sulfite Ion
An enzyme electrode was developed using the enzyme sulfite oxidase (EC1.8.3.1) immobilized onto pig intestine. Hydrogen peroxide produced was monitored amperometrically. All experimental parameters such as pH, temperature, buffer constituent concentration, were thoroughly investigated and optimized when appropriate. The response to sulfite concentration was linear in the range 5.2´...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Automatic Chemistry
دوره 11 شماره
صفحات -
تاریخ انتشار 1989